Počet záznamů: 1  

Use of Sindbis virus-mediated RNA interference to demonstrate a conserved role of Broad-Complex in insect metamorphosis

  1. 1.
    0136110 - ENTU-I 20033136 RIV US eng J - Článek v odborném periodiku
    Uhlířová, Miroslava - Foy, B. D. - Beaty, B. J. - Olson, K. E. - Riddiford, L. M. - Jindra, Marek
    Use of Sindbis virus-mediated RNA interference to demonstrate a conserved role of Broad-Complex in insect metamorphosis.
    Proceedings of the National Academy of Sciences of the United States of America. Roč. 100, č. 26 (2003), s. 15607-15612. ISSN 0027-8424
    Grant CEP: GA AV ČR IAA5007305
    Výzkumný záměr: CEZ:AV0Z5007907
    Klíčová slova: Green fluorescent protein-steroid-hormone ecdysone
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 10.272, rok: 2003

    The transcription factor Broad-Complex (BR-C) is required for differentiation of adult structures as well as for the programmed death of obsolete larval organs during metamorphosis of the fruit fly Drosophila melanogaster. Whether BR-C has a similar role in other holometabolous insects could not be proven without a loss-of-function genetic test, performed in a non-drosophilid species. Here we use a recombinant Sindbis virus as a tool to silence BR-C expression in the silkmoth Bombyx mori. The virus expressing a BR-C antisense RNA fragment reduced endogenous BR-C mRNA levels in infected tissues (adult wing and leg primordia) via RNA interference (RNAi). The RNAi knock-down of BR-C resulted in the failure of animals to complete the larval-pupal transition or in later morphogenetic defects, including differentiation of adult compound eyes, legs, and wings from their larval progenitors. BR-C RNAi also perturbed the programmed cell death of larval silk glands. These developmental defects correspond to loss-of-function phenotypes of BR-C Drosophila mutants in both the morphogenetic and degenerative aspects, suggesting that the critical role of BR-C in metamorphosis is evolutionarily conserved. We also demonstrate that the Sindbis virus is a useful vehicle for silencing of developmental genes in new insect models.
    Trvalý link: http://hdl.handle.net/11104/0033871