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Intracellular phosphorylation of benzyladenosine is related to apoptosis induction in tobacco BY-2 cells
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SYSNO ASEP 0104352 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Intracellular phosphorylation of benzyladenosine is related to apoptosis induction in tobacco BY-2 cells Překlad názvu Intracellular phosphorylation of benzyladenosine is related to apoptosis induction in tobacco BY-2 cells Tvůrce(i) Mlejnek, P. (CZ)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Procházka, S. (CZ)Zdroj.dok. Plant Cell and Environment. - : Wiley - ISSN 0140-7791
Roč. 26, č. 10 (2003), s. 1723-1735Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova adenosine 5'-triphosphate ; cytokinin ; N6-benzyladenine 9-riboside Vědní obor RIV ED - Fyziologie CEP LN00A081 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z5038910 - UEB-Q Anotace Treatment of tobacco BY-2 cells with micromolar concentration of benzyladenosine ([9R]BA) resulted in the loss of cell viability in a time- and concentration-dependent manner. Cell death induced by [9R]BA exhibited typical apoptotic hallmarks including cell shrinkage, chromatin condensation and degradation of nuclear DNA to characteristic high molecular weight (HMW) as well as nucleosomal size fragments. Externally added [9R]BA was very rapidly and almost quantitatively phosphorylated within BY-2 cells. Accumulation of [9R]BA-monophosphate was accompanied by massive production of endogenous reactive oxygen species (ROS), intracellular ATP depletion, and these events were followed by the loss of cell viability. Inhibition of intracellular phosphorylation of [9R]BA by adenosin kinase inhibitor, 5'-amino-5'-deoxyadenosine (AdAs), diminished ROS production, ATP depletion, and consequently prevented cells from death. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2005
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