Počet záznamů: 1  

Intracellular phosphorylation of benzyladenosine is related to apoptosis induction in tobacco BY-2 cells

  1. 1.
    0104352 - UEB-Q 20043015 RIV GB eng J - Článek v odborném periodiku
    Mlejnek, P. - Doležel, Jaroslav - Procházka, S.
    Intracellular phosphorylation of benzyladenosine is related to apoptosis induction in tobacco BY-2 cells.
    [Intracellular phosphorylation of benzyladenosine is related to apoptosis induction in tobacco BY-2 cells.]
    Plant Cell and Environment. Roč. 26, č. 10 (2003), s. 1723-1735. ISSN 0140-7791. E-ISSN 1365-3040
    Grant CEP: GA MŠk LN00A081
    Výzkumný záměr: CEZ:AV0Z5038910
    Klíčová slova: adenosine 5'-triphosphate * cytokinin * N6-benzyladenine 9-riboside
    Kód oboru RIV: ED - Fyziologie
    Impakt faktor: 3.613, rok: 2003

    Treatment of tobacco BY-2 cells with micromolar concentration of benzyladenosine ([9R]BA) resulted in the loss of cell viability in a time- and concentration-dependent manner. Cell death induced by [9R]BA exhibited typical apoptotic hallmarks including cell shrinkage, chromatin condensation and degradation of nuclear DNA to characteristic high molecular weight (HMW) as well as nucleosomal size fragments. Externally added [9R]BA was very rapidly and almost quantitatively phosphorylated within BY-2 cells. Accumulation of [9R]BA-monophosphate was accompanied by massive production of endogenous reactive oxygen species (ROS), intracellular ATP depletion, and these events were followed by the loss of cell viability. Inhibition of intracellular phosphorylation of [9R]BA by adenosin kinase inhibitor, 5'-amino-5'-deoxyadenosine (AdAs), diminished ROS production, ATP depletion, and consequently prevented cells from death.

    Intracellular phosphorylation of benzyladenosine is related to apoptosis induction in tobacco BY-2 cells.
    Trvalý link: http://hdl.handle.net/11104/0011626

     
     
Počet záznamů: 1  

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