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In vitro activation of CMV-specific human CD8ţ T cells by adenylate
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SYSNO ASEP 0377401 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název In vitro activation of CMV-specific human CD8ţ T cells by adenylate Tvůrce(i) Jelínek, J. (CZ)
Adkins, Irena (MBU-M)
Mikulková, Z. (CZ)
Jagosová, J. (CZ)
Pacasová, R. (CZ)
Michličková, S. (CZ)
Šebo, Peter (MBU-M) RID, ORCID
Michálek, J. (CZ)Zdroj.dok. Bone Marrow Transplantation - ISSN 0268-3369
Roč. 47, č. 2 (2012), s. 243-250Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova T cells ; antigenic peptide epitopes ; CyaA toxoid Vědní obor RIV EE - Mikrobiologie, virologie CEP 2B06161 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GP310/09/P582 GA ČR - Grantová agentura ČR CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000300415100013 DOI 10.1038/bmt.2011.68 Anotace Human CMV infects between 50-85% of healthy individuals and can cause live-threatening infections in immunocompromised patients. Therefore, peptide vaccination is being developed as a promising immunotherapeutic approach for treatment of patients at risk of CMV disease. The enzymatically inactive toxoid of Bordetella adenylate cyclase (CyaA-AC(-)) was shown to be an efficient tool for delivery of peptide epitopes and stimulation of Ag-specific T-cell immune responses. We investigated here the capacity of two CyaA-AC(-) constructs to deliver epitopes derived from the CMV phosphoprotein pp65 for activation of human T cells in vitro. Expansion of gamma-IFN-secreting CMV-specific CD8(+) T cells, as well as increase of total IFN-gamma and TNF-alpha production by PBMCs from CMV-seropositive donors were observed after in vitro stimulation with CyaA-AC(-) constructs carrying CMV epitopes, whereas limited activation of immune response occurred with free peptides Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2013
Počet záznamů: 1