In vitro activation of CMV-specific human CD8ţ T cells by adenylate

0377401 - MBÚ 2013 RIV GB eng J - Článek v odborném periodiku
Jelínek, J. - Adkins, Irena - Mikulková, Z. - Jagosová, J. - Pacasová, R. - Michličková, S. - Šebo, Peter - Michálek, J.
In vitro activation of CMV-specific human CD8ţ T cells by adenylate.
Bone Marrow Transplantation. Roč. 47, č. 2 (2012), s. 243-250. ISSN 0268-3369. E-ISSN 1476-5365
Grant CEP: GA MŠMT 2B06161; GA ČR GP310/09/P582
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: T cells * antigenic peptide epitopes * CyaA toxoid
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 3.541, rok: 2012

Human CMV infects between 50-85% of healthy individuals and can cause live-threatening infections in immunocompromised patients. Therefore, peptide vaccination is being developed as a promising immunotherapeutic approach for treatment of patients at risk of CMV disease. The enzymatically inactive toxoid of Bordetella adenylate cyclase (CyaA-AC(-)) was shown to be an efficient tool for delivery of peptide epitopes and stimulation of Ag-specific T-cell immune responses. We investigated here the capacity of two CyaA-AC(-) constructs to deliver epitopes derived from the CMV phosphoprotein pp65 for activation of human T cells in vitro. Expansion of gamma-IFN-secreting CMV-specific CD8(+) T cells, as well as increase of total IFN-gamma and TNF-alpha production by PBMCs from CMV-seropositive donors were observed after in vitro stimulation with CyaA-AC(-) constructs carrying CMV epitopes, whereas limited activation of immune response occurred with free peptides
Trvalý link: http://hdl.handle.net/11104/0209563