Autologous transplantation of mesenchymal stem cells into the portal vein of the miniature pig, a preliminary experiment for NOTES approach

0519194 - ÚŽFG 2020 RIV CZ eng J - Článek v odborném periodiku
Juhás, Štefan - Martínek, Jan - Ryska, Ondřej - Doležel, Radek - Ryska, Miroslav - Juhásová, Jana
Autologous transplantation of mesenchymal stem cells into the portal vein of the miniature pig, a preliminary experiment for NOTES approach.
Rozhledy v chirurgii. Roč. 98, č. 9 (2019), s. 350-355. ISSN 0035-9351
Grant CEP: GA MZd(CZ) NV16-27653A; GA MZd NV16-31806A; GA MŠMT(CZ) LO1609
Institucionální podpora: RVO:67985904
Klíčová slova: liver cell therapy * miniature pig * mesenchymal stem cells
Obor OECD: Transplantation
Způsob publikování: Omezený přístup
https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=18764053365

There is evidence that mesenchymal stem cells (MSCs) could trans-differentiate into the liver cells in vitro and in vivo and thus may be used as an unfailing source for stem cell therapy of liver disease. Combination of MSCs (with or without their differentiation in vitro) and minimally invasive procedures as laparoscopy or Natural Orifice Transluminal Endoscopic Surgery (NOTES) represents a chance for many patients waiting for liver transplantation in vain. METHODS: Over 30 millions of autologous MSCs at passage 3 were transplanted via the portal vein in an eight months old miniature pig. The deposition of transplanted cells in liver parenchyma was evaluated histologically and the trans-differential potential of CM-DiI labeled cells was assessed by expression of pig albumin using immunofluorescence. RESULTS: Three weeks after transplantation we detected the labeled cells (solitary, small clusters) in all 10 samples (2 samples from each lobe) but no diffuse distribution in the samples. The localization of CM-DiI+ cells was predominantly observed around the portal triads. We also detected the localization of albumin signal in CM-DiI labeled cells. CONCLUSION: The study results showed that the autologous MSCs (without additional hepatic differentiation in vitro) transplantation through the portal vein led to successful infiltration of intact miniature pig liver parenchyma with detectable in vivo trans-differentiation. NOTES as well as other newly developed surgical approaches in combination with cell therapy seem to be very promising for the treatment of hepatic diseases in near future.
Trvalý link: http://hdl.handle.net/11104/0304217