Unique Gene Expression Signatures in the Intestinal Mucosa and Organoids Derived from Germ-Free and Monoassociated Mice

0508126 - MBÚ 2020 RIV CH eng J - Článek v odborném periodiku
Janečková, L. - Kostovčíková, Klára - Švec, J. - Šťastná, M. - Strnad, H. - Kolář, M. - Hudcovic, Tomáš - Stančíková, J. - Turečková, J. - Baloghová, N. - Sloncová, E. - Galušková, K. - Tlaskalová-Hogenová, Helena - Kořínek, V.
Unique Gene Expression Signatures in the Intestinal Mucosa and Organoids Derived from Germ-Free and Monoassociated Mice.
International Journal of Molecular Sciences. Roč. 20, č. 7 (2019), č. článku 1581. E-ISSN 1422-0067
Grant CEP: GA ČR(CZ) GA16-06326S; GA ČR(CZ) GA19-08294S
Institucionální podpora: RVO:61388971
Klíčová slova: expression profiling * Enricher tool * microbiota
Obor OECD: Microbiology
Impakt faktor: 4.556, rok: 2019
Způsob publikování: Open access
https://www.mdpi.com/1422-0067/20/7/1581

Commensal microbiota contribute to gut homeostasis by inducing transcription of mucosal genes. Analysis of the impact of various microbiota on intestinal tissue provides an important insight into the function of this organ. We used cDNA microarrays to determine the gene expression signature of mucosa isolated from the small intestine and colon of germ-free (GF) mice and animals monoassociated with two E. coli strains. The results were compared to the expression data obtained in conventionally reared (CR) mice. In addition, we analyzed gene expression in colon organoids derived from CR, GF, and monoassociated animals. The analysis revealed that the complete absence of intestinal microbiota mainly affected the mucosal immune system, which was not restored upon monoassociation. The most important expression changes observed in the colon mucosa indicated alterations in adipose tissue and lipid metabolism. In the comparison of differentially expressed genes in the mucosa or organoids obtained from GF and CR mice, only six genes were common for both types of samples. The results show that the increased expression of the angiopoietin-like 4 (Angptl4) gene encoding a secreted regulator of lipid metabolism indicates the GF status.
Trvalý link: http://hdl.handle.net/11104/0299110