0580711 - ÚEM 2024 RIV DE eng J - Článek v odborném periodiku
Goncalves, A.M. - Leal, F. - Moreira, A. - Schellhorn, T. - Hefka Blahnová, Veronika - Zeiringer, S. - Vocetková, Karolína - Tetyczka, C. - Simaite, A. - Buzgo, M. - Roblegg, E. - Costa, P.F.F. - Ertl, P. - Filová, Eva - Kohl, Y.
Potential of Electrospun Fibrous Scaffolds for Intestinal, Skin, and Lung Epithelial Tissue Modeling.
Advanced NanoBiomed Research. Roč. 3, č. 4 (2023). ISSN 2699-9307
Grant CEP: GA MPO(CZ) FV40437
GRANT EU: European Commission(XE) 823981 - ActiTOX
Institucionální podpora: RVO:68378041
Klíčová slova: 3R principle * cellulose * electrospinning * epithelial barrier * in vitro modelst * toxicological screening
Obor OECD: Biomaterials (as related to medical implants, devices, sensors)
Způsob publikování: Open access
Web výsledku:
https://onlinelibrary.wiley.com/doi/10.1002/anbr.202200104DOI: https://doi.org/10.1002/anbr.202200104


Herein, intestinal, skin, and pulmonary in vitro tissue models based on electrospun membranes of poly(epsilon-caprolactone) (PCL) and cellulose acetate (CA), cellulose acetate phthalate (CAP), ethylcellulose (EC), or methylcellulose (MC) are presented. Physicochemical characterization and biocompatibility analyses of the scaffolds are carried out using colorectal adenocarcinoma cells (intestine), keratinocytes and fibroblasts (skin), and bronchial and alveolar epithelial cells (lung). PCL, PCL:CA, and PCL:EC are composed of nanofibers, whereas PCL:CAP and PCL:MC scaffolds comprise a combination of micro- and nanofibers. PCL, PCL:CA, PCL:CAP, and PCL:EC samples demonstrate water contact angles greater than 90 degrees and are, therefore, hydrophobic, while PCL:MC mats display a hydrophilic behavior. In intestinal models, cells adhere and proliferate on all scaffolds, in turn, studies with skin cell models reveal that PCL:CA and PCL:CAP blends outperform all other substrates. Lung cell models show that, while 16HBE cells adhere to and proliferate in PCL, PCL:CA, PCL:EC, and PCL:MC scaffolds, A549 cells only have the same biological response on PCL, PCL:CA, and PCL:MC. In summary, all fibrous meshes prepared are biocompatible toward most cell types tested, thus suggesting the potential of PCL-cellulose derivative blends as substrates suitable for in vitro epithelial tissue modeling and toxicity screening.
Trvalý link: https://hdl.handle.net/11104/0351158