Zooming In on Plant Hormone Analysis: Tissue- and Cell-Specific Approaches

0476573 - ÚEB 2018 RIV US eng J - Článek v odborném periodiku
Novák, Ondřej - Napier, R. - Ljung, K.
Zooming In on Plant Hormone Analysis: Tissue- and Cell-Specific Approaches.
Annual Review of Plant Biology. Roč. 68, č. 2017 (2017), s. 323-348. ISSN 1543-5008. E-ISSN 1545-2123
Grant CEP: GA MŠMT(CZ) LO1204
Institucionální podpora: RVO:61389030
Klíčová slova: Biosensor * Cell biology * Mass spectrometry * Phytohormone * Resolution * Sensitivity
Obor OECD: Plant sciences, botany
Impakt faktor: 18.712, rok: 2017

Plant hormones are a group of naturally occurring, low-abundance organic compounds that influence physiological processes in plants. Our knowledge of the distribution profiles of phytohormones in plant organs, tissues, and cells is still incomplete, but advances in mass spectrometry have enabled significant progress in tissue- and cell-type-specific analyses of phytohormones over the last decade. Mass spectrometry is able to simultaneously identify and quantify hormones and their related substances. Biosensors, on the other hand, offer continuous monitoring, can visualize local distributions and real-time quantification, and, in the case of genetically encoded biosensors, are noninvasive. Thus, biosensors offer additional, complementary technologies for determining temporal and spatial changes in phytohormone concentrations. In this review, we focus on recent advances in mass spectrometry-based quantification, describe monitoring systems based on biosensors, and discuss validations of the various methods before looking ahead at future developments for both approaches.
Trvalý link: http://hdl.handle.net/11104/0273048