A neo-functionalized homolog of host transmembrane protein controls localization of bacterial endosymbionts in the trypanosomatid Novymonas esmeraldas

0574476 - BC 2024 RIV US eng J - Článek v odborném periodiku
Zakharova, A. - Tashyreva, Daria - Butenko, Anzhelika - Morales, J. - Saura, A. - Svobodová, Michaela - Poschmann, G. - Nandipati, Satish - Zakharova, A. - Noyvert, D. - Gahura, Ondřej - Týč, Jiří - Stuehler, K. - Kostygov, A.Y. - Nowack, E.C.M. - Lukeš, Julius - Yurchenko, V.
A neo-functionalized homolog of host transmembrane protein controls localization of bacterial endosymbionts in the trypanosomatid Novymonas esmeraldas.
Current Biology. Roč. 33, č. 13 (2023), s. 2690-+. ISSN 0960-9822. E-ISSN 1879-0445
Grant CEP: GA ČR(CZ) GA20-07186S; GA MŠMT(CZ) EF16_013/0001775; GA MŠMT(CZ) EF16_019/0000759; GA MŠMT(CZ) LM2018129
Grant ostatní: Gordon and Betty Moore Foundation(US) #9354
Program: Science
Institucionální podpora: RVO:60077344
Klíčová slova: Trypanosomatidae * Leishmaniinae * endosymbiosis * Pandoraea * TMEM18
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 9.2, rok: 2022
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/pii/S0960982223005420?via%3Dihub

The stability of endosymbiotic associations between eukaryotes and bacteria depends on a reliable mechanism ensuring vertical inheritance of the latter. Here, we demonstrate that a host-encoded protein, located at the interface between the endoplasmic reticulum of the trypanosomatid Novymonas esmeraldas and its endosymbiotic bacterium Ca. Pandoraea novymonadis, regulates such a process. This protein, named TMP18e, is a product of duplication and neo-functionalization of the ubiquitous transmembrane protein 18 (TMEM18). Its expression level is increased at the proliferative stage of the host life cycle correlating with the confinement of bacteria to the nuclear vicinity. This is important for the proper segregation of bacteria into the daughter host cells as evidenced from the TMP18e ablation, which disrupts the nucleus-endosymbiont association and leads to greater variability of bacterial cell numbers, including an elevated proportion of aposymbiotic cells. Thus, we conclude that TMP18e is necessary for the reliable vertical inheritance of endosymbionts.
Trvalý link: https://hdl.handle.net/11104/0346008