Taxonomy of Aspergillus series Versicolores: species reduction and lessons learned about intraspecific variability

0566493 - MBÚ 2023 RIV NL eng J - Článek v odborném periodiku
Sklenář, František - Glässnerová, K. - Jurjević, Ž. - Houbraken, J. - Samson, R.A. - Visagie, C.M. - Yilmaz, N. - Gené, J. - Cano, J. - Chen, A.J. - Nováková, Alena - Yaguchi, T. - Kolařík, Miroslav - Hubka, Vít
Taxonomy of Aspergillus series Versicolores: species reduction and lessons learned about intraspecific variability.
Studies in Mycology. Roč. 102, November 16 (2022), s. 53-93. ISSN 0166-0616. E-ISSN 1872-9797
Grant CEP: GA MZd(CZ) NU21-05-00681
Institucionální podpora: RVO:61388971
Klíčová slova: Aspergillus creber * Aspergillus sydowii * Aspergillus versicolo * indoor fungi * multispecies coalescent model * osmotolerance, * species delimitation * sterigmatocystin
Obor OECD: Microbiology
Impakt faktor: 16.5, rok: 2022
Způsob publikování: Open access
https://www.studiesinmycology.org/index.php/issue/116-studies-in-mycology-no-102

Aspergillus series Versicolores members occur in a wide range of environments and substrates such as indoor environments, food, clinical materials, soil, caves, marine or hypersaline ecosystems. The taxonomy of the series has undergone numerous re-arrangements including a drastic reduction in the number of species and subsequent recovery to 17 species in the last decade. The identification to species level is however problematic or impossible in some isolates even using DNA sequencing or MALDI-TOF mass spectrometry indicating a problem in the definition of species boundaries. To revise the species limits, we assembled a large dataset of 518 strains. From these, a total of 213 strains were selected for the final analysis according to their calmodulin (CaM) genotype, substrate and geography. This set was used for phylogenetic analysis based on five loci (benA, CaM, RPB2, Mcm7, Tsr1). Apart from the classical phylogenetic methods, we used multispecies coalescence (MSC) model-based methods, including one multilocus method (STACEY) and five single-locus methods (GMYC, bGMYC, PTP, bPTP, ABGD). Almost all species delimitation methods suggested a broad species concept with only four species consistently
supported. We also demonstrated that the currently applied concept of species is not sustainable as there are incongruences between single-gene phylogenies resulting in different species identifications when using different gene regions. Morphological and physiological data showed overall lack of good, taxonomically informative characters, which could be used for identification of such a large number of existing species. The characters expressed either low variability across species or significant intraspecific variability exceeding interspecific variability.
Trvalý link: https://hdl.handle.net/11104/0337814