Počet záznamů: 1
Nucleotides Bearing Red Viscosity-Sensitive Dimethoxy-Bodipy Fluorophore for Enzymatic Incorporation and DNA Labeling
- 1.0566051 - ÚOCHB 2024 RIV US eng J - Článek v odborném periodiku
Kuba, Miroslav - Pohl, Radek - Kraus, Tomáš - Hocek, Michal
Nucleotides Bearing Red Viscosity-Sensitive Dimethoxy-Bodipy Fluorophore for Enzymatic Incorporation and DNA Labeling.
Bioconjugate Chemistry. Roč. 34, č. 1 (2023), s. 133-139. ISSN 1043-1802. E-ISSN 1520-4812
Grant CEP: GA ČR(CZ) GX20-00885X; GA MŠMT(CZ) EF16_019/0000729
Institucionální podpora: RVO:61388963
Klíčová slova: fluorescence * probes
Obor OECD: Organic chemistry
Impakt faktor: 4.7, rok: 2022
Způsob publikování: Omezený přístup
https://doi.org/10.1021/acs.bioconjchem.2c00547
Nucleosides and 2′-deoxyribonucleoside triphosphates (dNTPs) bearing 3,3′-dimethoxy-2,2′-diphenyl-6-(4-hydroxyphenyl)-bodipy fluorophore attached through a propargyl or propargyl-triethylene glycol linker to position 5 of 2′-deoxycytidine were designed and synthesized. They exerted bright red fluorescence and good sensitivity to viscosity changing their lifetime from 1.6 to 4.5 ns. The modifed dNTPs were substrates for DNA polymerases and were used in enzymatic synthesis of labeled DNA through primer extension. The modified DNA probes served as viscosity sensors responding to protein binding by changes of lifetime. The nucleotide with longer linker (dCpegMOBTP) was transported to live cells and incorporated into the genomic DNA, which can be useful for staining of DNA and imaging of DNA synthesis.
Trvalý link: https://hdl.handle.net/11104/0337489
Počet záznamů: 1