Microfluidic procedures for sample preparation, cell manipulation, and multidimensional separations

0558346 - ÚIACH 2023 CZ eng D - Dizertace
Václavek, Tomáš
Microfluidic procedures for sample preparation, cell manipulation, and multidimensional separations.
Ústav analytické chemie AV ČR, v. v. i. Obhájeno: Brno: Masarykova univerzita. 07.06.2022. - Brno: Masarykova univerzita, 2022. 145 s.
Institucionální podpora: RVO:68081715
Klíčová slova: biosensor * mass spectrometry * microfluidics * miniaturization * single-cell analysis
Obor OECD: Analytical chemistry

Modern analytical instruments provide ultra-low detection limits and utilize advanced and highly sensitive electronics. In line with the instrumental advances, miniaturization of sample delivery is an essential direction in analytical biochemistry. To operate with minute volumes of samples or reagents, miniaturized devices frequently exploit principles of microfluidics, a science of fluid manipulation at the microscale. This dissertation describes the development and applications of microfluidic and biosensor analytical instruments, including microfabrication strategies, micro-milling, and 3D printing. A substantial part of this thesis was dedicated to developing microfluidic tools for single-cell analysis. A microfluidic device that integrates the single-cell extraction from cell suspension, electrical lysis, buffer exchange, and nanospray ionization for mass spectrometry analysis of intracellular compounds was developed. Its performance was demonstrated by directly detecting hemoglobin alpha and beta chains released from a single erythrocyte. Another device designed to analyze single cells was a microfluidic flow-through sensor for counting and sizing particles and cells by resistive pulse sensing, which monitors the current disruptions caused by particles passing through a narrow pore. This device was applied to detect PC3 prostate cancer cells within a population of red blood cells. This work also presents microfluidic devices for interfacing liquid chromatography with capillary electrophoresis or a device for monitoring enzymatic reactions based on droplet microfluidics. Interdigitated gold microelectrodes for biosensor applications using electrochemical impedance spectroscopy and miniature biosensor casings for measurements in flow-through or droplet format, manufactured by micro-milling and 3D printing, are described as well.
Trvalý link: http://hdl.handle.net/11104/0332070