Počet záznamů: 1
The role of complement in the tick cellular immune defense against the entomopathogenic fungus Metarhizium robertsii
- 1.0553185 - BC 2022 RIV GB eng J - Článek v odborném periodiku
Fiorotti, J. - Urbanová, Veronika - Golo, P. - Elias Pinheiro Bittencourt, V. - Kopáček, Petr
The role of complement in the tick cellular immune defense against the entomopathogenic fungus Metarhizium robertsii.
Developmental and Comparative Immunology. Roč. 126, JAN 2022 (2022), č. článku 104234. ISSN 0145-305X. E-ISSN 1879-0089
Grant CEP: GA ČR(CZ) GA20-05736S
Institucionální podpora: RVO:60077344
Klíčová slova: rhipicephalus-microplus * ixodes-ricinus * biological-control * in-vitro * anisopliae * phagocytosis * pathogenicity * infection * hemolymph * ixodidae * Biological control * Entomopathogenic fungi * Hemocytes * Phagocytosis * Thioester-containing proteins * Tick
Obor OECD: Immunology
Impakt faktor: 2.9, rok: 2022
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/pii/S0145305X21002421?via%3Dihub
Entomopathogenic fungi (EPF) have been widely explored for their potential in the biological control of insect pests and as an environmentally friendly alternative to acaricides for limiting tick infestation in the field. The arthropod cuticle is the main barrier against fungal infection, however, an understanding of internal defense mechanisms after EPF intrusion into the invertebrate hemocoel is still rather limited. Using an infection model of the European Lyme borreliosis vector Ixodes ricinus with the EPF Metarhizium robertsii, we demonstrated that ticks are capable of protecting themselves to a certain extent against mild fungal infections. However, tick mortality dramatically increases when the capability of tick hemocytes to phagocytose fungal conidia is impaired. Using RNAi-mediated silencing of tick thioester-containing proteins (TEPs), followed by in vitro and/or in vivo phagocytic assays, we found that C3-like complement components and alpha 2-macroglobulin pan-protease inhibitors secreted to the hemolymph play pivotal roles in M. robertsii phagocytosis.
Trvalý link: http://hdl.handle.net/11104/0328186
Počet záznamů: 1