Molecular differences of adipose-derived mesenchymal stem cells between non-responders and responders in treatment of transphincteric perianal fistulas

0549766 - FGÚ 2022 RIV GB eng J - Článek v odborném periodiku
Tencerová, Michaela - Lundby, L. - Buntzen, S. - Norderval, S. - Hougaard, H. T. - Pedersen, B. G. - Kassem, M.
Molecular differences of adipose-derived mesenchymal stem cells between non-responders and responders in treatment of transphincteric perianal fistulas.
Stem Cell Research & Therapy. Roč. 12, č. 1 (2021), č. článku 586. E-ISSN 1757-6512
Institucionální podpora: RVO:67985823
Klíčová slova: autologous adipose tissue graft injection * transsphincteric perianal fistula * adipose-derived mesenchymal stem cells * stem cell potency * fistula healing
Obor OECD: Cell biology
Impakt faktor: 8.088, rok: 2021
Způsob publikování: Open access
https://doi.org/10.1186/s13287-021-02644-8

Background: Injection of autologous adipose tissue (AT) has recently been demonstrated to be an effective and safe treatment for anal fistulas. AT mesenchymal stem cells (AT-MSCs) mediate the healing process, but the relationship between molecular characteristics of AT-MSCs of the injected AT and fistula healing has not been adequately studied. Thus we aimed to characterize the molecular and functional properties of AT-MSCs isolated from autologous AT injected as a treatment of cryptogenic high transsphincteric perianal fistulas and correlate these findings to the healing process.Methods: 27 patients (age 45 +/- 2 years) diagnosed with perianal fistula were enrolled in the study and treated with autologous AT injected around the anal fistula tract. AT-MSCs were isolated for cellular and molecular analyses. The fistula healing was evaluated by MRI scanning after 6 months of treatment. AT-MSC phenotype was compared between responders and non-responders with respect to fistula healing.Results: 52% of all patients exhibited clinical healing of the fistulas as evaluated 6 months after last injection. Cultured AT-MSCs in the responder group had a lower short-term proliferation rate and higher osteoblast differentiation potential compared to non-responder AT-MSCs. On the other hand, adipocyte differentiation potential of AT-MSCs was higher in non-responder group. Interestingly, AT-MSCs of responders exhibited lower expression of inflammatory and senescence associated genes such as IL1B, NFKB, CDKN2A, TPB3,TGFB1.Conclusion: Our data suggest that cellular quality of the injected AT-MSCs including cell proliferation, differentiation capacity and secretion of proinflammatory molecules may provide a possible mechanism underlying fistula healing. Furthermore, these biomarkers may be useful to predict a positive fistula healing outcome.
Trvalý link: http://hdl.handle.net/11104/0325681