Global Genome Mining Reveals a Cytochrome P450-Catalyzed Cyclization of Crownlike Cyclodipeptides with Neuroprotective Activity

0547817 - MBÚ 2022 RIV US eng J - Článek v odborném periodiku
Malit, J. J. L. - Liu, W. - Cheng, A. - Saha, Subhasish - Liu, L-L. - Qian, P.-Y.
Global Genome Mining Reveals a Cytochrome P450-Catalyzed Cyclization of Crownlike Cyclodipeptides with Neuroprotective Activity.
Organic Letters. Roč. 23, č. 17 (2021), s. 6601-6605. ISSN 1523-7060. E-ISSN 1523-7052
Institucionální podpora: RVO:61388971
Klíčová slova: identification * biosynthesis
Obor OECD: Microbiology
Impakt faktor: 6.072, rok: 2021
Způsob publikování: Omezený přístup
https://pubs.acs.org/doi/10.1021/acs.orglett.1c01022

We conducted global genome mining of 162,672 bacterial genomes and identified 829 cyclodipeptide (CDP) biosynthesis gene clusters (BGC) containing a cytochrome P450 gene. Heterologous expression of BGC from Saccharopolyspora hirsuta DSM 44795 led to the identification of two novel crownlike CDPs, cyctetryptomycin A (4) and B (5), which possess unprecedented complex macrocycle and show neuroprotective activity. The two cytochrome P450s found in the BGC catalyze sequential reactions leading to the cyclization of diketopiperazine dimers.
Trvalý link: http://hdl.handle.net/11104/0323982