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Collagen Bioinks for Bioprinting: A Systematic Review of Hydrogel Properties, Bioprinting Parameters, Protocols, and Bioprinted Structure Characteristics

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    0546365 - ÚSMH 2022 RIV CH eng J - Článek v odborném periodiku
    Štěpanovská, J. - Šupová, Monika - Hanzálek, K. - Brož, Antonín - Matějka, R.
    Collagen Bioinks for Bioprinting: A Systematic Review of Hydrogel Properties, Bioprinting Parameters, Protocols, and Bioprinted Structure Characteristics.
    Biomedicines. Roč. 9, č. 9 (2021), č. článku 1137. E-ISSN 2227-9059
    Grant CEP: GA MZd(CZ) NV19-02-00068
    Institucionální podpora: RVO:67985891 ; RVO:67985823
    Klíčová slova: bioink * bioprinting * collagen * hydrogel * hydrogel properties * bioprinting parameters
    Obor OECD: Polymer science; Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction) (FGU-C)
    Impakt faktor: 4.757, rok: 2021
    Způsob publikování: Open access
    https://www.mdpi.com/2227-9059/9/9/1137

    Bioprinting is a modern tool suitable for creating cell scaffolds and tissue or organ carriers from polymers that mimic tissue properties and create a natural environment for cell development. A wide range of polymers, both natural and synthetic, are used, including extracellular matrix and collagen-based polymers. Bioprinting technologies, based on syringe deposition or laser technologies, are optimal tools for creating precise constructs precisely from the combination of collagen hydrogel and cells. This review describes the different stages of bioprinting, from the extraction of collagen hydrogels and bioink preparation, over the parameters of the printing itself, to the final testing of the constructs. This study mainly focuses on the use of physically crosslinked high-concentrated collagen hydrogels, which represents the optimal way to create a biocompatible 3D construct with sufficient stiffness. The cell viability in these gels is mainly influenced by the composition of the bioink and the parameters of the bioprinting process itself (temperature, pressure, cell density, etc.). In addition, a detailed table is included that lists the bioprinting parameters and composition of custom bioinks from current studies focusing on printing collagen gels without the addition of other polymers. Last but not least, our work also tries to refute the often-mentioned fact that highly concentrated collagen hydrogel is not suitable for 3D bioprinting and cell growth and development.
    Trvalý link: http://hdl.handle.net/11104/0322996

     
     
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