In situ hydroxyapatite synthesis enhances biocompatibility of pva/ha hydrogels

0546168 - ÚSMH 2022 RIV CH eng J - Článek v odborném periodiku
Chocholatá, P. - Kulda, V. - Dvořáková, J. - Šupová, Monika - Žaloudková, Margit - Babuška, V.
In situ hydroxyapatite synthesis enhances biocompatibility of pva/ha hydrogels.
International Journal of Molecular Sciences. Roč. 22, č. 17 (2021), č. článku 9335. E-ISSN 1422-0067
Institucionální podpora: RVO:67985891
Klíčová slova: In situ synthesis * Hydrogel * Scaffold * Polyvinyl alcohol * Hyaluronic acid * Hydroxyapatite * Bone tissue engineering * Biological evaluation * Cell viability
Obor OECD: Polymer science
Impakt faktor: 6.208, rok: 2021
Způsob publikování: Open access
https://www.mdpi.com/1422-0067/22/17/9335

Bone tissue engineering tries to simulate natural behavior of hard tissues. This study aimed to produce scaffolds based on polyvinyl alcohol (PVA) and hyaluronic acid (HA) with hy-droxyapatite (HAp) incorporated in two different ways, by in situ synthesis and physical mixing of pre-prepared HAp. In situ synthesis resulted in calcium deficient form of HAp with lower crystal-linity. The proliferation of human osteoblast-like cells MG-63 proved to be better in the scaffolds with in situ synthesized HAp compared to those with physically mixed pre-prepared HAp. For scaffolds with PVA/HA/HAp ratio 3:1:2, there was significantly higher initial adhesion (p = 0.0440), as well as the proliferation in the following days (p < 0.001). It seemed to be advantageous improve the properties of the scaffold by in situ synthesizing of HAp directly in the organic matrix.
Trvalý link: http://hdl.handle.net/11104/0322896