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Lipid Nanoparticles for Broad-Spectrum Nucleic Acid Delivery

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    0545469 - ÚOCHB 2022 RIV DE eng J - Článek v odborném periodiku
    Hejdánková, Zuzana - Vaněk, Václav - Sedlák, František - Procházka, Jan - Diederichs, Audrey - Kereiche, Sami - Novotná, Barbora - Buděšínský, Miloš - Birkuš, Gabriel - Grantz Šašková, Klára - Cígler, Petr
    Lipid Nanoparticles for Broad-Spectrum Nucleic Acid Delivery.
    Advanced Functional Materials. Roč. 31, č. 47 (2021), č. článku 2101391. ISSN 1616-301X. E-ISSN 1616-3028
    Grant CEP: GA MŠk(CZ) EF16_019/0000729; GA MŠk(CZ) LM2018126; GA MŠk EF16_013/0001789; GA MŠk EF18_046/0015861; GA MŠk ED2.1.00/19.0395; GA MŠk(CZ) ED1.1.00/02.0109
    Institucionální podpora: RVO:61388963 ; RVO:68378050
    Klíčová slova: cyclic dinucleotides * DNA * drug delivery * lipid nanoparticles * RNA
    Obor OECD: Biochemistry and molecular biology
    Impakt faktor: 19.924, rok: 2021
    Způsob publikování: Omezený přístup
    https://doi.org/10.1002/adfm.202101391

    Lipid nanoparticles (LNPs) are the most advanced nonviral modality for nucleic acid (NA) delivery, and have recently gained enormous attention in the fields of RNA therapeutics and vaccine development. Here, ionizable adamantane-based lipidoids named XMaNs, which circumvent the usual need for laborious optimization of LNP components for highly diverse types of NAs, are described. The non-toxic XMaN6 lipidoid is highly versatile in entrapment and delivery of siRNA, mRNA, plasmid DNA, and a cyclic dinucleotide. XMaN6-based LNPs efficiently deliver: 1) siRNA into human primary hepatocytes and cell lines that are hard-to-transfect, 2) mRNA into mouse liver, 3) plasmid DNA, 4) 2′,3′-cGAMP into cells and activated the cGAS-STING pathway three orders of magnitude more efficiently than 2′,3′-cGAMP alone. To our knowledge, such universality in delivering different NA types has not been previously described and can accelerate translation of LNPs into the clinic.
    Trvalý link: http://hdl.handle.net/11104/0322155

     
     
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