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Nanoscopic Structural Fluctuations of Disassembling Microtubules Revealed by Label-Free Super-Resolution Microscopy
- 1.0543280 - ÚFE 2022 RIV DE eng J - Článek v odborném periodiku
Vala, Milan - Bujak, Lukasz - Marín, Antonio García - Holanová, Kristýna - Henrichs, Verena - Braun, Marcus - Lánský, Zdeněk - Piliarik, Marek
Nanoscopic Structural Fluctuations of Disassembling Microtubules Revealed by Label-Free Super-Resolution Microscopy.
Small Methods. Roč. 5, č. 4 (2021), č. článku 2000985. ISSN 2366-9608. E-ISSN 2366-9608
Grant CEP: GA MŠMT(CZ) ED2.1.00/19.0390
Institucionální podpora: RVO:67985882 ; RVO:86652036
Klíčová slova: super-resolution microscopy * microtubules * dynamic instability * iSCAT
Obor OECD: Optics (including laser optics and quantum optics); Physical chemistry (BTO-N)
Impakt faktor: 15.367, rok: 2021
Způsob publikování: Open access
https://doi.org/10.1002/smtd.202000985
Microtubules are cytoskeletal polymers of tubulin dimers assembled into protofilaments that constitute nanotubes undergoing periods of assembly and disassembly. Static electron micrographs suggest a structural transition of straight protofilaments into curved ones occurring at the tips of disassembling microtubules. However, these structural transitions have never been observed and the process of microtubule disassembly thus remains unclear. Here, label-free optical microscopy capable of selective imaging of the transient structural changes of protofilaments at the tip of a disassembling microtubule is introduced. Upon induced disassembly, the transition of ordered protofilaments into a disordered conformation is resolved at the tip of the microtubule. Imaging the unbinding of individual tubulin oligomers from the microtubule tip reveals transient pauses and relapses in the disassembly, concurrent with increased organization of protofilament segments at the microtubule tip. These findings show that microtubule disassembly is a discrete process and suggest a stochastic mechanism of switching from the disassembly to the assembly phas
Trvalý link: http://hdl.handle.net/11104/0320513
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