Acetophenyl‐thienyl‐aniline‐Linked Nucleotide for Construction of Solvatochromic Fluorescence Light‐Up DNA Probes Sensing Protein‐DNA Interactions

0541740 - ÚOCHB 2022 RIV DE eng J - Článek v odborném periodiku
Güixens-Gallardo, Pedro - Hocek, Michal
Acetophenyl‐thienyl‐aniline‐Linked Nucleotide for Construction of Solvatochromic Fluorescence Light‐Up DNA Probes Sensing Protein‐DNA Interactions.
Chemistry - A European Journal. Roč. 27, č. 24 (2021), s. 7090-7093. ISSN 0947-6539. E-ISSN 1521-3765
Grant CEP: GA ČR(CZ) GA17-14791S; GA MŠMT(CZ) EF16_019/0000729
Grant ostatní: AV ČR(CZ) AP1501
Program: Akademická prémie - Praemium Academiae
Institucionální podpora: RVO:61388963
Klíčová slova: fluorescent labelling * DNA polymerases * nucleotides * protein-DNA interactions * solvatochromism
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 5.020, rok: 2021
Způsob publikování: Omezený přístup
https://doi.org/10.1002/chem.202100575

The synthesis of 2′‐deoxycytidine and its 5′‐O‐triphosphate bearing solvatochromic acetophenyl‐thienyl‐aniline fluorophore was developed using the Sonogashira cross‐coupling reaction as the key step. The triphosphate was used for polymerase synthesis of labelled DNA. The labelled nucleotide or DNA exerted weak red fluorescence when excited at 405 nm, but a significant colour change (to yellow or green) and light‐up (up to 20 times) was observed when the DNA probes interacted with proteins or lipids.
Trvalý link: http://hdl.handle.net/11104/0319263