Multiparametric flow cytometry analysis of peripheral blood B cell trafficking differences among Epstein-Barr virus infected and uninfected subpopulations

0537110 - MBÚ 2021 RIV CZ eng J - Článek v odborném periodiku
Zachová, K. - Kosztyu, P. - Zadražil, J. - Matoušovic, K. - Vondrák, K. - Hubáček, P. - Kostovčíková, Klára - Tlaskalová-Hogenová, Helena - Městecký, Jiří - Raška, M.
Multiparametric flow cytometry analysis of peripheral blood B cell trafficking differences among Epstein-Barr virus infected and uninfected subpopulations.
Biomedical Papers. Roč. 164, č. 3 (2020), s. 247-254. ISSN 1213-8118. E-ISSN 1804-7521
Grant CEP: GA MZd(CZ) NV15-33686A
Institucionální podpora: RVO:61388971
Klíčová slova: Epstein-Barr virus * cell trafficking * IgM * IgA * naive B cells
Obor OECD: Microbiology
Impakt faktor: 1.245, rok: 2020
Způsob publikování: Open access
https://biomed.papers.upol.cz/artkey/bio-202003-0004_multiparametric-flow-cytometry-analysis-of-peripheral-blood-b-cell-trafficking-differences-among-epstein-barr-v.php

Aims. Epstein-Barr virus (EBV) targets predominantly B cells and these cells could acquire new phenotype characteristics. Here we analyzed whether EBV-infected and -uninfected B cells from healthy subjects differ in proportion of dominant phenotypes, maturation stage, and homing receptors expression.
Methods. EBV-infected and -uninfected cells were identified by flow cytometry using fluorophore-labeled EBV RNA-specific DNA probes combined with fluorophore-labeled antibody to surface lineage markers, integrins, chemokine receptors, and immunoglobulin isotypes, including intracellular ones.
Results. Our results show that the trafficking characteristics of EBERpos B cells are distinct from EBERneg B cells with most dominant differences detected for alpha 4 beta 1 and alpha 4 beta 7 and CCR5 and CCR7. EBV-positive cells are predominantly memory IgM(+) B cells or plasmablasts/plasma cells (PB/PC) positive for IgA or less for IgM. In comparison to uninfected B cells, less EBV-positive B cells express alpha 4 beta 7 and almost no cells express alpha 4 beta 1. EBV-positive B cells contained significantly higher proportion of CCR5(+) and CCR7(+) cells in comparison to EBV-negative cells. In vitro exposure of blood mononuclear cells to pro-inflammatory cytokine IL-6 reduces population of EBV-positive B cell.
Conclusion. Although EBV-infected B cells represent only a minor subpopulation, their atypical functions could contribute in predisposed person to development abnormities such as some autoimmune diseases or tumors. Using multi-parameter flow cytometry we characterized differences in migration of EBV-positive and -negative B cells of various maturation stage and isotype of produced antibodies particularly different targeting to mucosal tissues of gastrointestinal and respiratory tracts.
Trvalý link: http://hdl.handle.net/11104/0314863