Effect of the anti-inflammatory drug diclofenac on lipid composition of bacterial strain Raoultella sp. KDF8

0531114 - MBÚ 2020 RIV CZ eng J - Článek v odborném periodiku
Palyzová, Andrea - Marešová, Helena - Novák, Jiří - Zahradník, Jiří - Řezanka, Tomáš
Effect of the anti-inflammatory drug diclofenac on lipid composition of bacterial strain Raoultella sp. KDF8.
Folia Microbiologica. Roč. 65, č. 4 (2020), s. 763-773. ISSN 0015-5632. E-ISSN 1874-9356
Grant CEP: GA MŠMT(CZ) LO1509; GA TA ČR TH02030337
Institucionální podpora: RVO:61388971
Klíčová slova: tandem mass-spectrometry * cyclopropane fatty-acids * escherichia-coli
Obor OECD: Microbiology
Impakt faktor: 2.099, rok: 2020
Způsob publikování: Omezený přístup
https://link.springer.com/content/pdf/10.1007/s12223-020-00790-9.pdf

The strain Raoultella sp. KDF8 was cultivated on three sources of carbon and energy, glycerol, ethanol and diclofenac, for periods of time ranging from 24 to 72 h. Using thin-layer chromatography, nine classes of phospholipids were detected and the amount of phosphatidylethanolamine (PtdEtn) decreased with increasing cultivation time. Conversely, the ratio of phospholipids having three or four acyls (acyl-phosphatidylglycerol (APtdGro), N-acyl-PtdEtn (NAPtdEtn) and cardiolipin (Ptd(2)Gro) increased during cultivation. GC-MS analysis showed that the percentage of fatty acids containing a cyclopropane ring increased almost tenfold whereas the amount of fatty acids bearing even-numbered chains dropped to less than one-third after 24 h and 72 h in cultures on glycerol and diclofenac, respectively. Shotgun analysis showed significant changes in the representation of molecular species of phospholipids. For instance, there was a 36-fold change in the ratio of 16:1/16:1/16:1-APtdGro to c17:0/c17:0/c17:0-APtdGro and a 12-fold ratio change for 16:1/16:1/16:1-NAPtdEtn to c17:0/c17:0/c17:0-NAPtdEtn, the Ptd(2)Gro ratio of 16:1 to c17:0 acids equalled 1750. Our results show that the bacteria overcome destabilization of the inner cytoplasmic cell membrane and a bacterial outer membrane by altering the geometric arrangement of acyl chains, i.e. switching from monounsaturated to cyclopropane fatty acids (16:1 versus c17:0).
Trvalý link: http://hdl.handle.net/11104/0309851