Počet záznamů: 1
Restricted and Non-Essential Redundancy of RNAi and piRNA Pathways in Mouse Oocytes
- 1.0521819 - ÚMG 2020 RIV US eng J - Článek v odborném periodiku
Táborská, Eliška - Pasulka, Josef - Malík, Radek - Horvat, Filip - Jeníčková, Irena - Matoševič, Z.J. - Svoboda, Petr
Restricted and Non-Essential Redundancy of RNAi and piRNA Pathways in Mouse Oocytes.
PLoS Genetics. Roč. 15, č. 12 (2019), č. článku e1008261. ISSN 1553-7404. E-ISSN 1553-7404
Grant CEP: GA MŠMT LO1419; GA MŠMT(CZ) LM2015040; GA MŠMT ED2.1.00/19.0395; GA MŠMT(CZ) ED1.1.00/02.0109; GA MŠMT(CZ) LM2015062; GA MŠMT(CZ) LM2015040; GA MŠMT ED2.1.00/19.0395
Grant ostatní: GA MŠk(CZ) LM2015042
Institucionální podpora: RVO:68378050
Klíčová slova: DOUBLE-STRANDED-RNA * GENE-EXPRESSION * RETROTRANSPOSON SUBFAMILY * MAMMALIAN OOCYTES * PIWI PROTEINS MILI * IDENTIFICATION * AMPLIFICATION * TRANSCRIPTION * INTERFERENCE
Obor OECD: Genetics and heredity (medical genetics to be 3)
Impakt faktor: 5.175, rok: 2019
Způsob publikování: Open access
https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1008261
Germline genome defense evolves to recognize and suppress retrotransposons. One of defensive mechanisms is the PIWI-associated RNA (piRNA) pathway, which employs small RNAs for sequence-specific repression. The loss of the piRNA pathway in mice causes male sterility while females remain fertile. Unlike spermatogenic cells, mouse oocytes posses also RNA interference (RNAi), another small RNA pathway capable of retrotransposon suppression. To examine whether RNAi compensates the loss of the piRNA pathway, we produced a new RNAi pathway mutant DicerSOM and crossed it with a catalytically-dead mutant of Mili, an essential piRNA gene. Normal follicular and oocyte development in double mutants showed that RNAi does not suppress a strong ovarian piRNA knock-out phenotype. However, we observed redundant and non-redundant targeting of specific retrotransposon families illustrating stochasticity of recognition and targeting of invading retrotransposons. Intracisternal A Particle retrotransposon was mainly targeted by the piRNA pathway, MaLR and RLTR10 retrotransposons were targeted mainly by RNAi. Double mutants showed accumulations of LINE-1 retrotransposon transcripts. However, we did not find strong evidence for transcriptional activation and mobilization of retrotransposition competent LINE-1 elements suggesting that while both defense pathways are simultaneously expendable for ovarian oocyte development, yet another transcriptional silencing mechanism prevents mobilization of LINE-1 elements.
Trvalý link: http://hdl.handle.net/11104/0306383
Název souboru Staženo Velikost Komentář Verze Přístup Plos_Genetics_E_Taborska_2019.pdf 2 2.6 MB Vydavatelský postprint vyžádat
Počet záznamů: 1