0508215 - ÚPT 2020 RIV GB eng J - Článek v odborném periodiku
Rebrošová, K. - Šiler, Martin - Samek, Ota - Růžička, F. - Bernatová, Silvie - Ježek, Jan - Zemánek, Pavel - Holá, V.
Identification of ability to form biofilm in Candida parapsilosis and Staphylococcus epidermidis by Raman spectroscopy.
Future Microbiology. Roč. 14, č. 6 (2019), s. 509-518. ISSN 1746-0913. E-ISSN 1746-0921
Grant CEP: GA ČR(CZ) GA15-20645S; GA MŠMT(CZ) LO1212; GA MŠMT ED0017/01/01
Institucionální podpora: RVO:68081731
Klíčová slova: biofilm * Candida parapsilosis * Raman spectroscopy * rapid diagnostics * Staphylococcus epidermidis
Obor OECD: Optics (including laser optics and quantum optics)
Impakt faktor: 2.907, rok: 2019
Způsob publikování: Omezený přístup
https://www.futuremedicine.com/doi/10.2217/fmb-2018-0297

Aim: Finding rapid, reliable diagnostic methods is a big challenge in clinical microbiology. Raman spectroscopy is an optical method used for multiple applications in scientific fields including microbiology. This work reports its potential in identifying biofilm positive strains of Candida parapsilosis and Staphylococcus epidermidis. Materials & methods: We tested 54 S. epidermidis strains (23 biofilm positive, 31 negative) and 51 C. parapsilosis strains (27 biofilm positive, 24 negative) from colonies on Mueller-Hinton agar plates, using Raman spectroscopy. Results: The accuracy was 98.9% for C. parapsilosis and 96.1% for S. epidermidis. Conclusion: Themethod showed great potential for identifying biofilm positive bacterial and yeast strains. We suggest that Raman spectroscopy might become a useful aid in clinical diagnostics.
Trvalý link: http://hdl.handle.net/11104/0299188