TAIL-LABELED OLIGONUCLEOTIDE PROBES FOR A DUAL ELECTROCHEMICAL MAGNETIC IMMUNOPRECIPITATION ASSAY OF DNA-PROTEIN BINDING

0506721 - BFÚ 2020 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Hermanová, Monika - Špaček, Jan - Orság, Petr - Fojta, Miroslav
TAIL-LABELED OLIGONUCLEOTIDE PROBES FOR A DUAL ELECTROCHEMICAL MAGNETIC IMMUNOPRECIPITATION ASSAY OF DNA-PROTEIN BINDING.
CECE 2014. 11th International Interdisciplinary Meeting on Bioanalysis. Brno: Institute of Analytical Chemistry AS CR, 2014 - (Foret, F.; Křenková, J.; Drobníková, I.; Guttman, A.; Klepárník, K.), Č. 2014 (2014), s. 74-76. ISBN 978-80-904959-2-0.
[CECE 2014. International Interdisciplinary Meeting on Bioanalysis /11./. Brno (CZ), 20.10.2014-22.10.2014]
Institucionální podpora: RVO:68081707
Klíčová slova: DNA-PROTEIN
Obor OECD: Analytical chemistry

A novel assay for detection of DNA-protein binding has been developed. Oligonucleotides bearing or lacking specific binding site of the p53 protein were tail-labeled by two different modified deoxynucleotide triphosphates using terminal deoxynucleotidyl transferase. Electrochemical detection enabled to discriminate between sequence-specific and non-specific p53-DNA binding in a competition assay.
Trvalý link: http://hdl.handle.net/11104/0297914