Anti-adhesive properties of nanocrystalline diamond films against Escherichia coli bacterium: Influence of surface termination and cultivation medium

0498303 - FZÚ 2019 RIV CH eng J - Článek v odborném periodiku
Budil, J. - Matyska Lišková, P. - Artemenko, Anna - Ukraintsev, Egor - Gordeev, Ivan - Beranová, J. - Konopásek, I. - Kromka, Alexander
Anti-adhesive properties of nanocrystalline diamond films against Escherichia coli bacterium: Influence of surface termination and cultivation medium.
Diamond and Related Materials. Roč. 83, Mar (2018), s. 87-93. ISSN 0925-9635. E-ISSN 1879-0062
Grant CEP: GA ČR GA15-01687S; GA MŠMT(CZ) LM2011026
Institucionální podpora: RVO:68378271
Klíčová slova: nanocrystalline diamond * termination * antibacterial * cultivation medium * Escherichia coli
Obor OECD: Biophysics
Impakt faktor: 2.290, rok: 2018

Nanocrystalline diamond (NCD) films were chosen as a promising material for characterizing their interaction with living systems due to their unique mechanical properties, chemical stability and biocompatibility. In this study, we examined the anti-adhesive properties of differently terminated NCD films deposited on glass or silicon against Escherichia coli bacterium. We focused on the influence of cultivation medium in this process and compared bacterial adhesion to H-, O- and F-terminated NCD films in complex Luria-Bertani (LB) and mineral (M9) cultivation media. We found the properties of NCD films to be independent of the base substrate. The anti-adhesive effect was observed only in the M9 medium where H- and F-terminated NCD films reduced bacterial adhesion by ca. 50%. In LB medium we did not observe the reduction of bacterial adhesion. For O-terminated NCD films, no anti-adhesive effect was observed, regardless of the cultivation medium. Our results show that the growth of Escherichia coli in M9 cultivation medium can be affected by the diamond atomic termination.
Trvalý link: http://hdl.handle.net/11104/0290854