Počet záznamů: 1  

ZNF143 protein is an important regulator of the myeloid transcription factor C/EBP

  1. 1. 0486300 - UMG-J 2018 RIV US eng J - Článek v odborném periodiku
    Gonzalez, D. - Luyten, A. - Bartholdy, B. - Zhou, Q. - Kardošová, Miroslava - Ebralidze, A. - Swanson, K.D. - Radomska, H.S. - Zhang, P. - Kobayashi, S.S. - Welner, R.S. - Levantini, E. - Steidl, U. - Chong, G. - Collombet, S. - Choi, M.H. - Friedman, A.D. - Scott, L.M. - Alberich-Jorda, Meritxell - Tenen, D.G.
    ZNF143 protein is an important regulator of the myeloid transcription factor C/EBP.
    Journal of Biological Chemistry. Roč. 292, č. 46 (2017), s. 18924-18936 ISSN 0021-9258
    Institucionální podpora: RVO:68378050
    Klíčová slova: CCAAT-enhancer-binding protein * gene regulation * hematopoiesis * promoter * transcription factor * EBPalpha * ZNF143
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Obor OECD: Cell biology
    Impakt faktor: 4.010, rok: 2017

    The transcription factor C/EBP is essential for myeloid differentiation and is frequently dysregulated in acute myeloid leukemia. Although studied extensively, the precise regulation of its gene by upstream factors has remained largely elusive. Here, we investigated its transcriptional activation during myeloid differentiation. We identified an evolutionarily conserved octameric sequence, CCCAGCAG, approximate to 100 bases upstream of the CEBPA transcription start site, and demonstrated through mutational analysis that this sequence is crucial for C/EBP expression. This sequence is present in the genes encoding C/EBP in humans, rodents, chickens, and frogs and is also present in the promoters of other C/EBP family members. We identified that ZNF143, the human homolog of the Xenopus transcriptional activator STAF, specifically binds to this 8-bp sequence to activate C/EBP expression in myeloid cells through a mechanism that is distinct from that observed in liver cells and adipocytes. Altogether, our data suggest that ZNF143 plays an important role in the expression of C/EBP in myeloid cells.
    Trvalý link: http://hdl.handle.net/11104/0281158