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Polyelectrolyte Complex Beads by Novel Two-Step Process for Improved Performance of Viable Whole-Cell Baeyer-Villiger Monoxygenase by Immobilization

  1. 1.
    0481790 - ÚPT 2018 RIV CH eng J - Článek v odborném periodiku
    Krajčovič, T. - Bučko, M. - Vikartovská, A. - Lacík, I. - Uhelská, L. - Chorvát, D. - Neděla, Vilém - Tihlaříková, Eva - Gericke, M. - Heinze, T. - Gemeiner, P.
    Polyelectrolyte Complex Beads by Novel Two-Step Process for Improved Performance of Viable Whole-Cell Baeyer-Villiger Monoxygenase by Immobilization.
    Catalysts. Roč. 7, č. 11 (2017), s. 353-364. ISSN 2073-4344
    Institucionální podpora: RVO:68081731
    Klíčová slova: polyelectrolyte complex beads * environmental scanning electron microscopy * confocal laser scanning microscopy * Baeyer-Villiger biooxidation * cyclohexanone monoxygenase * immobilization * viable whole-cell biocatalyst
    Kód oboru RIV: JA - Elektronika a optoelektronika, elektrotechnika
    Obor OECD: Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation
    Impakt faktor: 3.465, rok: 2017
    http://www.mdpi.com/2073-4344/7/11/353

    A novel immobilization matrix for the entrapment of viable whole-cell Baeyer–Villiger monooxygenase was developed. Viable recombinant Escherichia coli cells overexpressing cyclohexanone monooxygenase were entrapped in polyelectrolyte complex beads prepared by a two-step reaction of oppositely-charged polymers including highly defined cellulose sulphate. Immobilized cells exhibited higher operational stability than free cells during 10 repeated cycles of Baeyer–Villiger biooxidations of rac-bicyclo[3.2.0]hept-2-en-6-one to the corresponding lactones (1R,5S)-3-oxabicyclo-[3.3.0]oct-6-en-3-one and (1S,5R)-2-oxabicyclo-[3.3.0]oct-6-en-3-one. The morphology of polyelectrolyte complex beads was characterised by environmental scanning electron microscopy, the spatial distribution of polymers in the beads and cell viability were examined using confocal laser scanning microscopy, and the texture was characterised by the mechanical resistance measurements.
    Trvalý link: http://hdl.handle.net/11104/0277275
     
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