Počet záznamů: 1  

Atomic force spectroscopic and SPR kinetic analysis of long circular and short ssDNA molecules interacting with single-stranded DNA-binding protein

  1. 1. 0481141 - UIACH-O 2018 RIV AT eng J - Článek v odborném periodiku
    Horáčková, V. - Hlaváček, Antonín - Čundlerová, V. - Pastucha, M. - Skládal, P.
    Atomic force spectroscopic and SPR kinetic analysis of long circular and short ssDNA molecules interacting with single-stranded DNA-binding protein.
    Monatshefte fur Chemie. Roč. 148, č. 11 (2017), s. 2011-2018 ISSN 0026-9247
    Institucionální podpora: RVO:68081715
    Klíčová slova: microscopy * biology * specificity * surface
    Kód oboru RIV: CB - Analytická chemie, separace
    Obor OECD: Analytical chemistry
    Impakt faktor: 1.282, rok: 2016

    Regulation of cellular processes and biochemical pathways would not be possible without formation of specific non-covalent complexes between nucleic acids and proteins. Single-stranded DNA-binding proteins have a high affinity for ssDNA and this interaction plays a crucial role in the control of DNA replication, recombination, transcription, translation, and repair. Characterization of the DNA-protein interactions would improve the information about abnormal cells and provide a better understanding of tumor growth, its prevention, and medical treatment. The interaction between the ssDNA-binding protein from E. coli with two ssDNA molecules (either M13mp18, 7249 bases, or a short 10 base oligonucleotide) was analyzed using atomic force microscopy providing images of the formed complexes on mica. The corresponding binding forces were determined using force spectroscopy using cantilever tips modified with ssDNA. The interactions were also characterized using the surface plasmon resonance (Biacore) providing reference data on kinetics in real time. The data from different methods were critically evaluated and discussed with respect to correlation of the single- (force spectroscopy) and multi-molecular (biosensor kinetics) results.
    Trvalý link: http://hdl.handle.net/11104/0276740
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