Počet záznamů: 1
Assignment of sigma factors of RNA polymerase to promoters in Corynebacterium glutamicum
- 1. 0476299 - MBU-M 2018 RIV DE eng J - Článek v odborném periodiku
Dostálová, Hana - Holátko, Jiří - Busche, T. - Rucká, Lenka - Rapoport, Andrey - Halada, Petr - Nešvera, Jan - Kalinowski, J. - Pátek, Miroslav
Assignment of sigma factors of RNA polymerase to promoters in Corynebacterium glutamicum.
AMB Express. Roč. 7, JUN 23 (2017), s. 1-13, č. článku 133. ISSN 2191-0855
Grant CEP: GA ČR(CZ) GA17-06991S
Institucionální podpora: RVO:61388971
Klíčová slova: Corynebacterium glutamicum * Promoter * Sigma factor
Kód oboru RIV: EE - Mikrobiologie, virologie
Obor OECD: Microbiology
Impakt faktor: 1.719, rok: 2017
Corynebacterium glutamicum is an important industrial producer of various amino acids and other metabolites. The C. glutamicum genome encodes seven sigma subunits (factors) of RNA polymerase: the primary sigma factor SigA (sA), the primary-like sigma(B) and five alternative sigma factors (sigma(C), sigma(D), sigma(E), sigma(H) and sigma(M)). We have developed in vitro and in vivo methods to assign particular sigma factors to individual promoters of different classes. In vitro transcription assays and measurements of promoter activity using the overexpression of a single sigma factor gene and the transcriptional fusion of the promoter to the gfpuv reporter gene enabled us to reliably define the sigma factor dependency of promoters. To document the strengths of these methods, we tested examples of respective promoters for each C. glutamicum sigma factor. Promoters of the rshA (anti-sigma for sH) and trxB1 (thioredoxin) genes were found to be sigma(H)-dependent, whereas the promoter of the sigB gene (sigma factor sigma(B)) was sigma(E)-and sigma(H)-dependent. It was confirmed that the promoter of the cg2556 gene (iron-regulated membrane protein) is sigma(C)-dependent as suggested recently by other authors. The promoter of cmt1 (trehalose corynemycolyl transferase) was found to be clearly sigma(D)-dependent. No sigma(M)-dependent promoter was identified. The typical housekeeping promoter P2sigA (sigma factor sigma(A)) was proven to be sigma(A)-dependent but also recognized by sigma(B). Similarly, the promoter of fba (fructose-1,6-bisphosphate aldolase) was confirmed to be sigma(B)-dependent but also functional with sigma(A). The study provided demonstrations of the broad applicability of the developed methods and produced original data on the analyzed promoters.
Trvalý link: http://hdl.handle.net/11104/0272843