0472227 - ÚŽFG 2017 RIV GB eng J - Článek v odborném periodiku
Anýz, J. - Vysloužilová, L. - Vaculovič, T. - Tvrdoňová, M. - Kanický, V. - Haase, H. - Horák, Vratislav - Štěpánková, O. - Heger, Z. - Adam, V.
Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration.
Scientific Reports. Roč. 7, č. 1 (2017), č. článku 40169. ISSN 2045-2322. E-ISSN 2045-2322
Grant CEP: GA MŠMT(CZ) LO1609
Institucionální podpora: RVO:67985904
Klíčová slova: melanoma patients * mixed effects models * bearing Libechov minipig
Obor OECD: Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions (pharmacogenomics, gene-based therapeutics)
Impakt faktor: 4.122, rok: 2017

We describe a new procedure for the parallel mapping of selected metals in histologically characterized tissue samples. Mapping is achieved via image registration of digital data obtained from two neighbouring cryosections by scanning the first as a histological sample and subjecting the second to laser ablation inductively coupled plasma mass spectrometry. This computer supported procedure enables determination of the distribution and content of metals of interest directly in the chosen histological zones and represents a substantial improvement over the standard approach, which determines these values in tissue homogenates or whole tissue sections. The potential of the described procedure was demonstrated in a pilot study that analysed Zn and Cu levels in successive development stages of pig melanoma tissue using MeLiM (Melanoma-bearing-Libechov-Minipig) model. We anticipate that the procedure could be useful for a complex understanding of the role that the spatial distribution of metals plays within tissues affected by pathological states including cancer.
Trvalý link: http://hdl.handle.net/11104/0269536