0467109 - MBÚ 2017 RIV US eng J - Článek v odborném periodiku
Górska, S. - Hermanová, Petra - Ciekot, J. - Schwarzer, Martin - Šrůtková, Dagmar - Brzozowska, E. - Kozáková, Hana - Gamian, A.
Chemical characterization and immunomodulatory properties of polysaccharides isolated from probiotic Lactobacillus casei LOCK 0919.
Glycobiology. Roč. 26, č. 9 (2016), s. 1014-1024. ISSN 0959-6658. E-ISSN 1460-2423
Institucionální podpora: RVO:61388971
Klíčová slova: immunomodulation * Lactobacillus * NMR spectroscopy
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 3.112, rok: 2016

The Lactobacillus casei strain, LOCK 0919, is intended for the dietary management of food allergies and atopic dermatitis (LATOPIC(A (R)) BIOMED). The use of a probiotic to modulate immune responses is an interesting strategy for solving imbalance problems of gut microflora that may lead to various disorders. However, the exact bacterial signaling mechanisms underlying such modulations are still far from being understood. Here, we investigated variations in the chemical compositions and immunomodulatory properties of the polysaccharides (PS), L919/A and L919/B, which are produced by L. casei LOCK 0919. By virtue of their chemical features, such PS can modulate the immune responses to third-party antigens. Our results revealed that L919/A and L919/B could both modulate the immune response to Lactobacillus planatarum WCFS1, but only L919/B could alter the response of THP-1 cells (in terms of tumor necrosis factor alpha production) to L. planatarum WCFS1 and Escherichia coli Nissle 1917. The comprehensive immunochemical characterization is crucial for the understanding of the biological function as well as of the bacteria-host and bacteria-bacteria cross-talk.
Trvalý link: http://hdl.handle.net/11104/0265359