0466928 - MBÚ 2017 RIV SK eng J - Článek v odborném periodiku
Frimmelová, M. - Toman, R. - Pompach, Petr - Škultéty, L'udovít
Modifications in the glycerophospholipid composition between the Coxiella burnetii phase I and phase II cells suggest an association with phase variation of the bacterium.
Acta virologica. Roč. 60, č. 1 (2016), s. 27-33. ISSN 0001-723X. E-ISSN 1336-2305
Grant CEP: GA MŠMT(CZ) LO1509; GA MŠMT(CZ) EE2.3.30.0003; GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:61388971
Klíčová slova: Coxiella burnetii * glycerophospholipids * FT-ICR MS
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 0.673, rok: 2016

Glycerophospholipids (GP) extracted from the Coxiella burnetii strain Nine Mile in virulent phase I (NM I) and low virulent phase II (NM II) were analyzed by Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) that gave a superior mass resolution and mass accuracy allowing unambiguous peak recognition and precise assignment of ions. We showed that GP present in the pathogen's outer membrane underwent considerable modifications during the phase variation that might be related to impact of various environmental factors. It was found that GP from phase I cells were much more complex than those from phase II cells. While glycerophosphoethanolamines (PE), glycerophosphocholines (PC) and glycerophosphoglycerols (PG) were present in both phases of C. burnetii, major differences were observed in the presence of glycerophosphates (PA) and glycerophosphoserines (PS). Thus, PA but no PS were detected in NM I variant in contrast with NM II cells where PS but no PA were identified. It is suggested that enzymes for PA head group modifications to form PS, PE, and PG become active during the phase variation of the bacterium.
Trvalý link: http://hdl.handle.net/11104/0265361