Počet záznamů: 1
Modified Nucleic Acids
- 1.0461380 - ÚOCHB 2017 RIV CH eng M - Část monografie knihy
Daďová, Jitka - Cahová, Hana - Hocek, Michal
Polymerase Synthesis of Base-Modified DNA.
Modified Nucleic Acids. Cham: Springer, 2016 - (Nakatani, K.; Tor, Y.), s. 123-144. Nucleic Acids and Molecular Biology, 31. ISBN 978-3-319-27109-5
Institucionální podpora: RVO:61388963
Klíčová slova: enzyme amplification reaction * cross-coupling reactions * labeled nucleoside triphosphonates
Kód oboru RIV: CC - Organická chemie
Enzymatic synthesis of base-modified DNA by polymerase incorporation of modified nucleotides is discussed. Modified 20-deoxyribonucleoside triphosphates (dNTPs) are key substrates for polymerases and can be prepared either by triphosphorylation of modified nucleosides or by direct aqueous cross-coupling reactions of halogenated dNTPs with alkynes, arylboronic acids, or alkenes. The methods of polymerase synthesis include primer extension, PCR, nicking enzyme amplifications, and other methods which enable the synthesis of diverse types of long or short and double-stranded DNA or single-stranded oligonucleotides. The applications include labeling in diagnostics (labeling or coding of DNA bases) and chemical biology (bioconjugations, modulation of protein binding, etc.).
Trvalý link: http://hdl.handle.net/11104/0260996
Počet záznamů: 1