Počet záznamů: 1
A population of giant tailed virus-like particles associated with heterotrophic flagellates in a lake-type reservoir
- 1. 0450149 - BC-A 2016 RIV DE eng J - Článek v odborném periodiku
Weinbauer, M.G. - Dolan, J. R. - Šimek, Karel
A population of giant tailed virus-like particles associated with heterotrophic flagellates in a lake-type reservoir.
Aquatic Microbial Ecology. Roč. 76, č. 2 (2015), s. 111-116 ISSN 0948-3055
Grant CEP: GA ČR(CZ) GA13-00243S
Institucionální podpora: RVO:60077344
Klíčová slova: viral infection * virus induced mortality * burst size * heterotrophic flagellates
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 2.109, rok: 2015
Using transmission electron microscopy (TEM), a population of giant virus-like particles (VLPs) with a head diameter of ca. 405 nm and a flexible ca. 1100 nm long tail was detected in a lake-type reservoir. These giant VLPs were abundant in situ at the start of a survey period (3.3 × 104 particles ml−1) and increased by 7-fold within 96 h. This VLP population vanished in dialysis bag incubations of 0.8 μm-filtered reservoir water (free of bacterivorous flagellates) but increased markedly in the enhanced bacterivory treatment, i.e. 5 μm filtered water. In the latter, incubation, heterotrophic nanoflagellate (HNF) abundance increased approximately 15-fold during the study. A multiple regression analysis using microbial abundances and grazing rates as parameters indicated that 78% of the variability in the abundance of giant VLPs was explained by HNF abundance and grazing rates. Our data support the hypothesis that this virus population infects flagellates. Observation of a presumptive lysing flagellate cell suggests a viral burst size of 15. Estimations of decay and net production rates from dialysis bag incubations indicate that lysis due to giant viruses could cause between 10 to 60% of the mortality of the total flagellate community and, thus, viruses are potentially a significant factor shaping the population dynamics of fl a gellates in freshwater.
Trvalý link: http://hdl.handle.net/11104/0251651