Počet záznamů: 1  

Prolyl Oligopeptidase from the Blood Fluke Schistosoma mansoni: From Functional Analysis to Anti-schistosomal Inhibitors

  1. 1. 0446340 - UOCHB-X 2016 RIV US eng J - Článek v odborném periodiku
    Fajtová, Pavla - Štefanic, S. - Hradilek, Martin - Dvořák, Jan - Vondrášek, Jiří - Jílková, Adéla - Ulrychová, Lenka - McKerrow, J. H. - Caffrey, C. R. - Mareš, Michael - Horn, Martin
    Prolyl Oligopeptidase from the Blood Fluke Schistosoma mansoni: From Functional Analysis to Anti-schistosomal Inhibitors.
    PLoS Neglected Tropical Diseases. Roč. 9, č. 6 (2015), e0003827/1-e0003827/24 ISSN 1935-2735
    Grant CEP: GA ČR(CZ) GAP302/11/1481; GA MŠk LO1302
    Institucionální podpora: RVO:61388963 ; RVO:68378050
    Klíčová slova: Schistosoma mansoni * schistosomiasis * prolyl oligopeptidase * blood fluke
    Kód oboru RIV: CE - Biochemie; EB - Genetika a molekulární biologie (UMG-J)
    Impakt faktor: 3.948, rok: 2015

    Blood flukes of the genus Schistosoma cause schistosomiasis, a parasitic disease that infects over 240 million people worldwide, and for which there is a need to identify new targets for chemotherapeutic interventions. Our research is focused on Schistosoma mansoni prolyl oligopeptidase (SmPOP) from the serine peptidase family S9, which has not been investigated in detail in trematodes. We demonstrate that SmPOP is expressed in adult worms and schistosomula in an enzymatically active form. By immunofluorescence microscopy, SmPOP is localized in the tegument and parenchyma of both developmental stages. Recombinant SmPOP was produced in Escherichia coli and its active site specificity investigated using synthetic substrate and inhibitor libraries, and by homology modeling. SmPOP is a true oligopeptidase that hydrolyzes peptide (but not protein) substrates with a strict specificity for Pro at P1. The inhibition profile is analogous to those for mammalian POPs. Both the recombinant enzyme and live worms cleave host vasoregulatory, proline-containing hormones such as angiotensin I and bradykinin. Finally, we designed nanomolar inhibitors of SmPOP that induce deleterious phenotypes in cultured schistosomes. We provide the first localization and functional analysis of SmPOP together with chemical tools for measuring its activity. We briefly discuss the notion that SmPOP, operating at the host-parasite interface to cleave host bioactive peptides, may contribute to the survival of the parasite. If substantiated, SmPOP could be a new target for the development of anti-schistosomal drugs.
    Trvalý link: http://hdl.handle.net/11104/0248346