Počet záznamů: 1  

Ms1, a novel sRNA interacting with the RNA polymerase core in mycobacteria

  1. 1.
    0439950 - MBU-M 2015 RIV GB eng J - Článek v odborném periodiku
    Hnilicová, Jarmila - Jirát-Matějčková, Jitka - Šiková, Michaela - Pospíšil, Jiří - Halada, Petr - Pánek, Josef - Krásný, Libor
    Ms1, a novel sRNA interacting with the RNA polymerase core in mycobacteria.
    Nucleic Acids Research. Roč. 42, č. 18 (2014), s. 11763-11776. ISSN 0305-1048
    Grant CEP: GA ČR(CZ) GBP305/12/G034; GA ČR GP13-27150P
    Grant ostatní:Magistrát hl. m. P.(CZ) CZ.2.16/3.1.00/24023
    Institucionální podpora: RVO:61388971
    Klíčová slova: ESCHERICHIA-COLI * 6S RNA * NONCODING RNA
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 9.112, rok: 2014

    Small RNAs (sRNAs) are molecules essential for a number of regulatory processes in the bacterial cell. Here we characterize Ms1, a sRNA that is highly expressed in Mycobacterium smegmatis during stationary phase of growth. By glycerol gradient ultracentrifugation, RNA binding assay, and RNA co-immunoprecipitation, we show that Ms1 interacts with the RNA polymerase (RNAP) core that is free of the primary sigma factor (sigma(A)) or any other sigma factor. This contrasts with the situation in most other species where it is 6S RNA that interacts with RNAP and this interaction requires the presence of sigma(A). The difference in the interaction of the two types of sR-NAs (Ms1 or 6S RNA) with RNAP possibly reflects the difference in the composition of the transcriptional machinery between mycobacteria and other species. Unlike Escherichia coli, stationary phase M. smegmatis cells contain relatively few RNAP molecules in complex with sigma(A). Thus, Ms1 represents a novel type of small RNAs interacting with RNAP.
    Trvalý link: http://hdl.handle.net/11104/0243161