0436077 - MBÚ 2015 RIV US eng J - Článek v odborném periodiku
Rocha, B. A. - de Oliveira, A. M. - Pazin, M. - Dorta, D.J. - Rodrigues, A.P.N. - Berretta, A.A. - Peti, A. P. F. - de Moraes, L.A.B. - Lopes, N. P. - Pospíšil, Stanislav - Gates, P. J. - Assis, M. D.
Jacobsen Catalyst as a Cytochrome P450 Biomimetic Model for the Metabolism of Monensin A.
BioMed Research International. Roč. 2014, č. 2014 (2014), s. 1-8. ISSN 2314-6133. E-ISSN 2314-6141
Institucionální podpora: RVO:61388971
Klíčová slova: Monensin A * Jacobsen Catalyst
Obor OECD: Microbiology
Impakt faktor: 1.579, rok: 2014 ; AIS: 0.367, rok: 2014
DOI: https://doi.org/10.1155/2014/152102

Monensin A, an important antibiotic ionophore, is employed to treat coccidiosis and displays a variety of biological properties. In this study, the Jacobsen catalyst as a cytochrome P450 biomimetic model was used to investigate the oxidation of monensin A. Mass spectrometry analysis of the products from these model systems revealed the formation of two products: 3-O-demethyl monensin A and 12-hydroxy monensin A which are the same ones found in in vivo models. Monensin A and products obtained in biomimetic model were tested in a mitochondrial toxicity model assessment and an antimicrobial bioassay against Staphylococcus aureus, S. aureus methicillin-resistant, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Escherichia coli. The results demonstrated the toxicological effects of monensin A in isolated rat liver mitochondria but not its products, showing that the metabolism of monensin A is a detoxification process . In addition, the antimicrobial bioassay showed that monensin A and its products possessed activity against Gram-positive microorganisms but not for Gram-negative microorganisms.
Trvalý link: http://hdl.handle.net/11104/0239925