0435675 - ÚOCHB 2015 RIV NL eng J - Článek v odborném periodiku
Chamieh, J. - Koval, Dušan - Besson, A. - Kašička, Václav - Cottet, H.
Generalized polymer effective charge measurement by capillary isotachophoresis.
Journal of Chromatography A. Roč. 1370, Nov 28 (2014), s. 255-262. ISSN 0021-9673. E-ISSN 1873-3778
Grant CEP: GA ČR(CZ) GA13-17224S; GA MŠMT 7AMB12FR012
Grant ostatní: GA AV ČR(CZ) M200551207
Institucionální podpora: RVO:61388963
Klíčová slova: polymer effective charge * polyelectrolyte * isotachophoresis * counter-ion condensation * capillary electrophoresis
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 4.169, rok: 2014

In this work, we have generalized the use of capillary isotachophoresis as a universal method for determination of effective charge of anionic and cationic (co)polymers on ordinary capillary electrophoresis instruments. This method is applicable to a broad range of strong or weak polyelectrolytes with good repeatability. Experimental parameters (components and concentrations of leading and terminating electrolytes, capillary diameters, constant electric current intensity) were optimized for implementation in 100 mu m i.d. capillaries for both polyanions and polycations. Determined values of polymer effective charge were in a very good agreement with those obtained by capillary electrophoresis with indirect UV detection. Uncertainty of the effective charge measurement using isotachophoresis was addressed and estimated to be similar to 5-10% for solutes with mobilities in the 20-50 x 10(-9) m(2) V-1 s(-1) range.
Trvalý link: http://hdl.handle.net/11104/0239683