0435172 - BFÚ 2015 RIV US eng J - Článek v odborném periodiku
Juda, P. - Šmigová, J. - Kováčik, L. - Bártová, Eva - Raška, I.
Ultrastructure of Cytoplasmic and Nuclear Inosine-5 '-Monophosphate Dehydrogenase 2 "Rods and Rings" Inclusions.
Journal of Histochemistry & Cytochemistry. Roč. 62, č. 10 (2014), s. 739-750. ISSN 0722-186X
Grant CEP: GA ČR(CZ) GBP302/12/G157
Institucionální podpora: RVO:68081707
Klíčová slova: Inosine-5-monophosphate dehydrogenase * inhibitors of IMPDH * Rods and Rings
Kód oboru RIV: BO - Biofyzika
DOI: https://doi.org/10.1369/0022155414543853

Inosine-5-monophosphate dehydrogenase catalyzes the critical step in the de novo synthesis of guanosine nucleotides: the oxidation of inosine monophosphate to xanthosine monophosphate. This reaction can be inhibited by specific inhibitors, such as ribavirin or mycophenolic acid, which are widely used in clinical treatment when required to inhibit the proliferation of viruses or cells. However, it was recently found that such an inhibition affects the cells, leading to a redistribution of IMPDH2 and the appearance of IMPDH2 inclusions in the cytoplasm. According to their shape, these inclusions have been termed Rods and Rings (R&R). In this work, we focused on the subcellular localization of IMPDH2 protein and the ultrastructure of R&R inclusions. Using microscopy and western blot analysis, we show the presence of nuclear IMPDH2 in human cells.
Trvalý link: http://hdl.handle.net/11104/0239097