Physical and biocatalytic properties of polyvinyl alcohol lens-shaped particles versus spherical polyelectrolyte complex microcapsules

0434640 - ÚPT 2015 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Schenkmayerová, A. - Bučko, M. - Gemeiner, P. - Treľová, D. - Lacík, I. - Chorvát Jr., D. - Ačai, P. - Polakovič, M. - Lipták, L. - Rebroš, M. - Rosenberg, M. - Štefuca, V. - Neděla, Vilém - Tihlaříková, Eva
Physical and biocatalytic properties of polyvinyl alcohol lens-shaped particles versus spherical polyelectrolyte complex microcapsules.
Workshop of Interesting Topics of SEM and ESEM. Brno: Institute of Scientific Instruments AS CR, v. v. i, 2014, s. 38-39. ISBN 978-80-87441-12-1.
[Workshop of Interesting Topics of SEM and ESEM. Mikulov (CZ), 26.08.2014-31.08.2014]
Grant CEP: GA MŠMT EE.2.3.20.0103
Institucionální podpora: RVO:68081731
Klíčová slova: SEM * ESEM
Kód oboru RIV: JA - Elektronika a optoelektronika, elektrotechnika

Direct comparison of key physical and chemical-engineering properties of two representative matrices for multipurpose immobilisations was performed for the first time. Polyvinyl alcohol lens-shaped particles LentiKats and polyelectrolyte complex microcapsules were characterised by advanced techniques with respect to the size distribution of the particles, their inner morphology as revealed by fluorescent probe staining, mechanical resistance, size-exclusion properties, determination of effective diffusion coefficient and environmental scanning electron microscope imaging. Recombinant cells E. coli overexpressing enzyme cyclopentanone monooxygenase were immobilised in polyelectrolyte complex microcapsules and LentiKats for comparison of their operational stability using model Baeyer-Villiger oxidation of (±)-cis-bicyclo[3.2.0]hept-2-en-6-one to regioisomeric lactones as important chiral synthons for potential pharmaceuticals.
Trvalý link: http://hdl.handle.net/11104/0238653