Počet záznamů: 1  

Nuclear track-based biosensors with the enzyme laccase

  1. 1. 0432984 - UJF-V 2015 RIV NL eng J - Článek v odborném periodiku
    Garcia-Arellano, H. - Fink, Dietmar - Hernandez, G. M. - Vacík, Jiří - Hnatowicz, Vladimír - Alfonta, L.
    Nuclear track-based biosensors with the enzyme laccase.
    Applied Surface Science. Roč. 310, SI (2014), s. 66-76 ISSN 0169-4332
    Grant CEP: GA ČR(CZ) GBP108/12/G108; GA MŠk(XE) LM2011019
    Institucionální podpora: RVO:61389005
    Klíčová slova: Biosensor * Laaccase * nuclear tracks * Phenolic compounds
    Kód oboru RIV: BM - Fyzika pevných látek a magnetismus
    Impakt faktor: 2.711, rok: 2014

    A new type of biosensors for detecting phenolic compounds is presented here. These sensors consist of thin polymer foils with laccase-clad etched nuclear tracks. The presence of suitable phenolic compounds in the sensors leads to the formation of enzymatic reaction products in the tracks, which differ in their electrical conductivities from their precursor materials. These differences correlate with the concentrations of the phenolic compounds. Corresponding calibration curves have been established for a number of compounds. The sensors thus produced are capable to cover between 5 and 9 orders of magnitude in concentration in the best case down to some picomoles. The sensor's detection sensitivity strongly depends on the specific compound. It is highest for caffeic acid and acid blue 74, followed by ABTS and ferulic acid.
    Trvalý link: http://hdl.handle.net/11104/0237280