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How to Preserve Plant Samples for Carbohydrate Analysis? Test of Suitable Methods Applicable in Remote Areas

  1. 1.
    0432919 - BÚ 2015 RIV NL eng J - Článek v odborném periodiku
    Chlumská, Zuzana - Janeček, Štěpán - Doležal, Jiří
    How to Preserve Plant Samples for Carbohydrate Analysis? Test of Suitable Methods Applicable in Remote Areas.
    Folia Geobotanica. Roč. 49, č. 1 (2014), s. 1-15. ISSN 1211-9520
    Grant CEP: GA AV ČR IAA600050802; GA ČR GA526/09/0963; GA ČR GD206/08/H044; GA ČR GA526/07/0808
    Institucionální podpora: RVO:67985939
    Klíčová slova: ethanol extraction * liquid nitrogen * nonstructural carbohydrates
    Kód oboru RIV: EF - Botanika
    Impakt faktor: 1.778, rok: 2014

    Normally the plants tissues subjected to nonstructural carbohydrate analyses are stored in Dewar flasks with liquid nitrogen. This cannot be applied in remote areas, such as high mountains, because flasks are very heavy and liquid nitrogen evaporates in a few weeks. We invented a new method, which is much more suitable for field conditions - plant tissues can only be boiled in hot 50% ethanol, using simple equipment. This fully ensures denaturation of enzymes and plants tissues can be easily transported in small test tubes and can be stored for more than a month.
    Trvalý link: http://hdl.handle.net/11104/0237251