Počet záznamů: 1  

In vitro Culture of a Novel Genotype of Ehrlichia sp from Brazil

  1. 1. 0421276 - BC-A 2014 RIV DE eng J - Článek v odborném periodiku
    Zweygarth, E. - Schol, H. - Lis, K. - Cabezas Cruz, Alejandro - Thiel, C. - Silaghi, C. - Ribeiro, M.F.B. - Passos, L.M.F.
    In vitro Culture of a Novel Genotype of Ehrlichia sp from Brazil.
    Transboundary and Emerging Diseases. Roč. 60, NOV 2013 (2013), s. 86-92 ISSN 1865-1674
    Grant ostatní:EU(XE) FP7-PEOPLE-ITN No.238511
    Institucionální podpora: RVO:60077344
    Klíčová slova: Ehrlichia * Rhipicephalus (Boophilus) microplus * in vitro culture * tick cell * DH82 * endothelial cell * cattle * 16S rRNA * Brazil
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 3.116, rok: 2013

    Ehrlichiae are obligate intracytoplasmic Gram-negative, tick-borne bacteria belonging to the Anaplasmataceae family. Ehrlichioses are considered emerging diseases in both humans and animals. Several members of the genus Ehrlichia have been isolated and propagated in vitro. This study describes the continuous propagation of a Brazilian Ehrlichia sp. isolate in IDE8 tick cells, canine DH82 cells and bovine aorta cells. Initially, the organisms were isolated from the haemolymph of a Rhipicephalus (Boophilus) microplus tick into IDE8 cells. Infected IDE8 cells were brought from Brazil to Germany, where the organisms were continuously propagated in IDE8, DH82 and bovine aorta cells. Bovine aorta cells were infected and propagated for 3months, corresponding to six subcultures, whereas the other two infected cell lines were kept for more than 1year. During the cultivation period, 36 and 14 subcultures were carried out in IDE8 and DH82 cell cultures, respectively. Reinfection of IDE8 cells with organisms grown in DH82 cells was achieved. Sequence analysis made with a fragment of the 16S rRNA gene showed that this Ehrlicha sp. is closely related to Ehrlichia canis. However, the maximum likelihood phylogenetic tree shows that it falls in a separate phylogenetic clade from E.canis.
    Trvalý link: http://hdl.handle.net/11104/0227657