Počet záznamů: 1  

Towards crystal structures of antibodies and transcription factors

  1. 1. 0398525 - UOCHB-X 2014 RIV CZ eng J - Článek v odborném periodiku
    Písačková, Jana - Procházková, Kateřina - Král, Vlastimil - Fábry, Milan - Řezáčová, Pavlína
    Towards crystal structures of antibodies and transcription factors.
    Materials Structure in Chemistry, Biology, Physics and Technology. Roč. 20, č. 2 (2013), s. 121-123 ISSN 1211-5894
    Grant CEP: GA MŠk ME08016; GA MŠk 1M0505; GA ČR GA203/09/0820
    Výzkumný záměr: CEZ:AV0Z40550506; CEZ:AV0Z50520514
    Klíčová slova: scFv antibody fragment * DeoR repressor protein * thermofluor assay * protein crystallization
    Kód oboru RIV: FR - Farmakologie a lékárnická chemie

    Protein three-dimensional structure determination by X-ray crystallography is important for understanding protein function. The protein crystallization bottleneck can be overcome by optimization of factors which influence the protein crystallizability. We demonstrated this approach on two scFv antibody fragments, and on prokaryotic metabolic regulator. We have performed protein pre-crystallization analysis employing the combination of size-exclusion chromatography, dynamic light scattering, and thermofluor assay. Based on the analysis data, we have developed crystallization protocols which yielded monocrystals suitable for X-ray diffraction analysis. This enabled us to determine crystal structure of the effector-binding domain of DeoR repressor.
    Trvalý link: http://hdl.handle.net/11104/0226021