Estrogen receptors in mouse testis and sperm

0394649 - BTÚ 2014 CA eng A - Abstrakt
Dostálová, Pavla - Děd, Lukáš - Pěknicová, Jana
Estrogen receptors in mouse testis and sperm.
Abstracts. Montréal: Society for the Study of Reproduction, 2013 - (Suarez, S.). s. 340-340
[SSR 46th Annual Meeteing. Reproduction Health: Nano to Global. 22.07.2013-26.07.2013, Montréal]
Grant CEP: GA ČR(CZ) GAP503/12/1834
Výzkumný záměr: CEZ:AV0Z50520701
Klíčová slova: Estrogen receptor α * Estrogen receptor β * Reproduction * Spermatogenesis
Kód oboru RIV: EB - Genetika a molekulární biologie

In males, estrogens are produced mainly in testicular tissue and play an important role in male reproduction. Generally, estrogens mediate their effect through two different pathways – genomic which leads to specific estrogen-dependent gene expression (male reproductive tissue) and non-genomic which occurs much faster and involves second messengers (male reproductive tissue, sperm). Nevertheless, receptors involvement is expected in both cases. Two types of classical ERs are known – ERα and ERβ. In addition to classical ERs, there exist membrane-localized ERs and splice variants with different DNA- and ligand-binding properties. This study aims to contribute to better understanding of the estrogen signaling in males. Therefore, we focus on the identification and characterization of classical ERs and their splice variants in mouse testicular cells and sperm. According to our results, it seems that ERα occurs only in its full-length variant in testicular cells, while ERβ occurs at least in two splice variants. To going deeper, we prepared testicular cell suspension and using FACS we separated cells according to their ploidity. RNA was isolated and used for further analysis, in order to reveal whether the various stages of germ cells express the same variant of ER or whether there are some changes among different testicular cell types. With regard to the sperm, we localized the signal in acrosomal region and in the tail for both ERs in immunofluorescence study. As in testicular cells, we detected several bands in the sperm cell lysate in western blot study, which probably indicates the presence of several splice variants also in sperm. Taking together, our results of protein and mRNA detection support the hypothesis that not only full-length ERs are expressed in the mouse testis and sperm, and that the mechanism of estrogen action in male reproduction is more complex than previously considered.
Trvalý link: http://hdl.handle.net/11104/0223154