Počet záznamů: 1  

Lipid alterations in human colon epithelial cells induced to differentiation and/or apoptosis by butyrate and polyunsaturated fatty acids

  1. 1. 0389673 - BFU-R 2013 RIV US eng J - Článek v odborném periodiku
    Hofmanová, Jiřina - Ciganek, M. - Slavík, J. - Kozubík, Alois - Stixová, Lenka - Vaculová, Alena - Dušek, L. - Machala, M.
    Lipid alterations in human colon epithelial cells induced to differentiation and/or apoptosis by butyrate and polyunsaturated fatty acids.
    Journal of Nutritional Biochemistry. Roč. 23, č. 6 (2012), s. 539-548 ISSN 0955-2863
    Grant CEP: GA ČR(CZ) GA524/07/1178; GA ČR(CZ) GAP301/11/1730
    Výzkumný záměr: CEZ:AV0Z50040507
    Institucionální podpora: RVO:68081707
    Klíčová slova: Colon cancer * Polyunsaturated fatty acids * Butyrate
    Kód oboru RIV: BO - Biofyzika
    Impakt faktor: 4.552, rok: 2012

    The present study highlights the important association between lipid alterations and differentiation/apoptotic responses in human colon differentiating (FHC) and nondifferentiating (HCT-116) cell lines after their treatment with short-chain fatty acid sodium butyrate (NaBt), polyunsaturated fatty acids (PUFAs), and/or their combination. Our data from GC/MS and LC/MS/MS showed an effective incorporation and metabolization of the supplemented arachidonic acid (AA) or docosahexaenoic acid (DNA), resulting in an enhanced content of the respective PUFA in individual phospholipid (PL) classes and an altered composition of the whole cellular fatty acid spectrum in both FHC and HCT-116 cells. We provide novel evidence that NaBt combined with PUFAs additionally modulated AA and DHA cellular levels and caused their shift from triacylglycerol to PL fractions.
    Trvalý link: http://hdl.handle.net/11104/0218526