0385220 - BTÚ 2013 RIV GB eng J - Článek v odborném periodiku
Čapková, Jana - Hrnčíř, Tomáš - Kubátová, Alena - Tlaskalová-Hogenová, Helena
Lipopolysaccharide treatment suppresses spontaneously developing ankylosing enthesopathy in B10.BR male mice: The potential role of interleukin-10.
BMC Musculoskeletal Disorders. Roč. 13, č. 6 (2012). E-ISSN 1471-2474
Grant CEP: GA ČR(CZ) GAP304/11/1252; GA ČR GP310/09/P182; GA MŠMT(CZ) 7E09091
Výzkumný záměr: CEZ:AV0Z50520701
Institucionální podpora: RVO:61388971
Klíčová slova: Ankylosing enthesopathy * Interleukin-10 * Lipopolysacharid * Cytokines
Kód oboru RIV: EC - Imunologie
Impakt faktor: 1.875, rok: 2012

Ankylosing enthesopathy (ANKENT) is an animal model of human ankylosing spondylitis. ANKENT is an inflammatory disease affecting the ankle and tarsal joints of the hind limbs in susceptible mouse strains. In the disease, the participation of intestinal microbiota components was suggested. Therefore, we attempted to increase the incidence of ANKENT by systemic administration of lipopolysaccharide (LPS), which is a component of bacterial cellular walls and stimulates inflammatory processes. ANKENT occurrence, serum cytokine profiles, spleen cellular composition and in vitro cytokine response to LPS were analysed in LPS-treated and control LPS-untreated B10.BR male mice. Contrary to expectations, LPS treatment decreased the incidence of ANKENT in LPS-treated group compared to control LPS-untreated group. Flow cytometry analysis of splenocytes showed an increased percentage of macrophages, dendritic cells and neutrophils and a decreased percentage of B cells, T cells and T helper cells in LPS-treated males following LPS administration. In addition, LPS-treated males had significantly elevated IL-6 and IL-10 serum levels. At 20-22 weeks after the final LPS application, splenocytes from LPS-treated mice were more susceptible to in vitro LPS stimulation than those of the controls and produced significantly higher levels of TNF alpha and IL-6. Repeated systemic stimulation with microbial component lipopolysaccharide in early adulthood significantly reduced the incidence of ANKENT in B10.BR mice and this finding can support the "hygiene hypothesis". In LPS-treated mice, the innate immunity parameters and the level of anti-inflammatory IL-10 cytokine were significantly increased. Nevertheless, the immunological mechanism of the LPS protective effect remains
Trvalý link: http://hdl.handle.net/11104/0219957