0385037 - FZÚ 2013 RIV US eng J - Článek v odborném periodiku
Meziane, D. - Barras, A. - Kromka, Alexander - Houdková, Jana - Boukherroub, R. - Szunerits, S.
Thiol-yne reaction on boron-doped diamond electrodes: application for the electrochemical detection of DNA-DNA hybridization events.
Analytical Chemistry. Roč. 84, č. 1 (2012), s. 194-200. ISSN 0003-2700. E-ISSN 1520-6882
Grant CEP: GA AV ČR KAN400100701
Výzkumný záměr: CEZ:AV0Z10100521
Klíčová slova: boron doped diamond * electrochemistry * surface functionalization
Kód oboru RIV: BM - Fyzika pevných látek a magnetismus
Impakt faktor: 5.695, rok: 2012

Boron-doped diamond (BDD) interfaces were chemically functionalized through the catalyst free thiol-yne reaction. Different thiolated precursors (e.g., perfluorodecanethiol, 6-(ferrocenyl)-hexanethiol, DNA) were successfully “clicked” to alkynyl-terminated BDD by irradiating the interface at 365 nm for 30 min. Thiolated oligonucleotide strands were immobilized using the optimized reaction conditions, and the surface concentration was tuned to obtain a surface coverage of 3.1 × 1012 molecules cm–2. Electrochemical impedance spectroscopy (EIS) was employed to follow the kinetics of hybridization and dehybridization events. The sensitivity of the oligonucleotide modified BDD interface was assayed, and a detection limit of 1 nM was obtained.
Trvalý link: http://hdl.handle.net/11104/0214444